Read e-book online Affinity labeling PDF

By Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek

ISBN-10: 0121819469

ISBN-13: 9780121819460

The seriously acclaimed laboratory normal, Methods in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless proper this day - actually a necessary book for researchers in all fields of lifestyles sciences

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Biophys. 144, 723 (1971). ~IK. A. Koehler and G. P. Hess, Biochemistry 13, 5345 (1974). 22S. Cha, R. P. Agarwal, and R. E. , Biochem. , in press. ~sK. D. Collins, J. Biol. Chem. 49, 136 (1974). ~"G. E. Lienhard and I. I. Secemski, J. Biol. Chem. 248, 1121 (1973). 28 Although binding ratios as large as 105-106 are found in some cases, it should be emphasized that true Ks values are not often known for productive ES complexes, and may be higher or lower than the observed K~ values on which the apparent binding ratios are based.

144, 723 (1971). ~IK. A. Koehler and G. P. Hess, Biochemistry 13, 5345 (1974). 22S. Cha, R. P. Agarwal, and R. E. , Biochem. , in press. ~sK. D. Collins, J. Biol. Chem. 49, 136 (1974). ~"G. E. Lienhard and I. I. Secemski, J. Biol. Chem. 248, 1121 (1973). 28 Although binding ratios as large as 105-106 are found in some cases, it should be emphasized that true Ks values are not often known for productive ES complexes, and may be higher or lower than the observed K~ values on which the apparent binding ratios are based.

Wolfenden, J. Biol. Chem. 249, 6351 (1974). r j. D. Findlater and B. A. Orsi, FEBS Left. $6, 109 (1973) ° J. Conchie, A. J. Hay, I. Strachan, and G. A. Levvy, Biochem. J. 102, 929 (1967). t It. L. Lai and B. Axelrod, Biochem. Biophys. Res. Commun. 64, 463 (1973). B. E. Evans and R. Wolfenden, J. Am. Chem. Soc. 92, 4751 (1970). P. W. K. Woo, It. W. Dion, S. M. Lange, L. F. Dahl, and L. J. Durham, J. Helerocycl. Chem. 11, 641 (1974). R. M. Cohen and R. Wolfenden, J. Biol. Chem. 246, 7561 (1971). L.

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Affinity labeling by Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek


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