By M L Anson; John T Edsall
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Extra info for Advances in protein chemistry. / Volume
Further work is required to establish the reasons for these disagreements, Lazarow (1943) and Barron (1943) discuss the r61e of mitochondria and microsomes as carriers for enzymes involved in cellular metabolism. (1 BTRUCPORAL PROTEINS OF CELLS AND "ISSUES 31 R6k of Protein Particulates in Cellular Enzyme Reactions. In addition to the components mentioned above, various high molecular weight particles having enzymic activity have been isolated (see particularly Stern, 1939, 1943; Korr, 1939; Henle and Chambers, 1940; and Iiabat, 1941).
If the asymmetric submicroscopic particles are oriented with long axes perpendicular to the optic axis, then In the biological cam nr may be varied by immersing the system in media of Merent refractive indices and mewring the birefringence (n, - q ) after sufficient time haa been allowed for penetration of each medium. The general practice is to plot n, noaa ordinatea against nn aa a b s c i e . The result is ueually a paraboloid c u m , 88 shown in Fig. 4. - It has been recognized, but not always remembered, that positive or negative form birefringence need not mean that the submicroscopic particles are rodlets or platelets, respectively.
3% P. Like microsomes they are ribonucleoproteipphospholipid complexes, the lipid portion representing 20-24% of the total. The d-amino acid oxidme is said to be localized in these granules, none being in the microsome fraction. Claude also finds (personal communication) that practically all of the extractable succinic dehydrogenase and cytochrome oxidase are associated with the secretion granules, very little being present in microsomes. Transaminase activity is also associated with these granules but not with the microsomes.
Advances in protein chemistry. / Volume by M L Anson; John T Edsall