Read e-book online Advances in Biochemical Engineering Biotechnology, Volume PDF

By W. Babel, A. Steinbuechel (Editor)

ISBN-10: 3540411410

ISBN-13: 9783540411413

Residing structures synthesize seven various sessions of polymers. they supply constitution and shape for cells and organisms, functionality as catalysts and effort garage and hold the genetic details. these kinds of polymers own technically fascinating houses. a few of these biopolymers are already used commercially. This precise quantity of Advances in Biochemical Engineering/Biotechnology includes 10 chapters. It supplies an summary of the water insoluble biopolyesters, specifically of the microbially synthesized poly-hydroxyalkanoate (PHA) relatives. It stories the state-of-the-art of metabolism, law and genetic historical past, the newest advances made in genetic optimization of micro organism, "construction" of transgenic crops and in vitro synthesis via purified enzymes. moreover, it describes correct applied sciences and evaluates views relating expanding the commercial viability and competitiveness of PHA and discusses purposes in medication, packaging, foodstuff and different fields.

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Solani f. pisi with the 5¢-flanking region of cutinase gene fused to a reporter gene, chloramphenicol acetyl transferase (CAT). Deletion and mutation of the various segments of the cutinase gene and a measurement of the effects of such alterations on the promoter activity revealed several important regulatory elements in the cutinase promoter [173]. A G-rich region at –340 bp that is needed for inducible expression, a silencer element at –287 bp that keeps cutinase gene repressed until induced, and a palindromic region at –170 bp that is absolutely essential for transcriptional activation by the cutin monomers were identified.

US Pat 5,545, 547 Lin T-S, Kolattukudy PE (1980) Physiol Plant Pathol 17:1 Fett WF, Wijey C, Moreau RA, Osman SF (1999) J Applied Microbiol 86:561 Heinen W, De Vries H (1966) Arch Mikrobiol 54:331 Heinen W, De Vries H (1966) Arch Mikrobiol 54:339 Shishiyama J, Araki F, Akai S (1970) Plant Cell Physiol 11:937 van den Ende G, Linskens HF (1974) Ann Rev Phytopathol 12:247 Purdy RE, Kolattukudy PE (1975) Biochemistry 14:2824 Kolattukudy PE, Purdy RE, Maiti IB (1981) Cutinases from fungi and pollen.

E. Kolattukudy consensus element. CRE2, located at –160 to –181, is a direct repeat resembling the thyroid hormone response element. These elements were concatenated and were used to screen expression libraries. This approach yielded a clone that encodes a protein that binds CRE1. This CRE1 binding protein (CREBP) may be a steroid receptor-like protein and, if so, the regulation of cutinase gene expression by the hydrophobic cutin monomers may bear analogy to gene transcriptional activation by steroid hormones in mammals.

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Advances in Biochemical Engineering Biotechnology, Volume 071, Biopolyesters by W. Babel, A. Steinbuechel (Editor)

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