By J. M. Foidart, A. Noel, H. Emonard, B. Nusgens, Ch. M. Lapiere (auth.), Alain O. A. Miller (eds.)
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Extra resources for Advanced Research on Animal Cell Technology
Miller I presume that the cDNA has been made for these Would it be experimentally feasible cells with the cDNA from to proteins. transfect HeLa such gap junctions proteins and make these HeLa cells which you said were lacking the gap junctions now respond to such things Hulser Indeed, I forgot to mention that LOEWENSTEIN's group done similar experiments with the messenger able to induce gap junctions gap junctions. yet been done. RNA and in cells which did not With cDNA this type of experiment has has was have not FIBRONECTIN-MEDIATED CELLULAR (INTER)ACTIONS B.
1982, 1984; Urushihara and Yamada, 1986). 46 These possible receptors are less well characterized than the RGD receptors but are potentially important to explain the specificity in the interactions of cells with fibronectin. 2_ The RGD-fibronectin receptor Two main approaches have independently led to the identification of a complex of 140 kDa glycoproteins as the cellular receptor for fibronectin. An immunological approach was based on the assumption that antibodies raised against surface antigens only interfere with adhesion of cells to substrata if they react with molecules directly involved in the adhesive process (see fig.
However, by comparing the regulatory functions of gap junctions under in vivo conditions, with primary cell cultures and with permanently growing cellines, the in vitro growth conditions have been improved. These studies helped to demonstrate the capability of threedimensionally growing multicell spheroids and may lead to successful mass cultivations of mammalian cells with high yields of cell production. ) Bennett, M. C. L. and Goodenough, D. ) Gap junctions, electrotonic coupling, and intercellular communication In: Neurosciences Research Program Bulletin 16, 372 - 486, MIT Press, 1978 Berdan, R.
Advanced Research on Animal Cell Technology by J. M. Foidart, A. Noel, H. Emonard, B. Nusgens, Ch. M. Lapiere (auth.), Alain O. A. Miller (eds.)